Western blot analysis of DLAT in different various lysates. Lane 1: SK-Br-3 cell lysate; Lane 2: K562 cell lysate; Lane 3: Daudi cell lysate; Lane 4: HL-60 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 69 kDa. Observed band size: 69 kDa. Exposure time: 2 minutes; 8% SDS-PAGE gel. Primary antibody DLAT recombinant monoclonal antibody (Product # MA5-50773) with a dilution of 1:500 was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG-HRP Secondary Antibody with a dilution of 1:300,000 was used for 1 hour at room temperature. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature.